Tb green® premix ex taqtm ii kit
WebTakara SYBR Green Master Mix products contain TaKaRa Ex Taq HS DNA Polymerase, a hot start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq … WebDec 21, 2024 · RNA of L. cubeba was extracted and reverse transcribed by the method provided by Zhao et al. (2024). qRT-PCR was carried out with the assistance of an ABI PRISM 7500 instrument and the TB Green ® Premix Ex TaqTM II kit.
Tb green® premix ex taqtm ii kit
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WebTB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration … WebFeb 1, 2024 · TB Green® Premix Ex TaqTM II (Tli RNase H Plus) was used for real-time PCR analysis performed on Applied Biosystems (Thermo Fisher Scientific, Waltham, MA, USA). The following primers were used: F 5′-TCCTTCAGACACCCTCAAC-3′, R 5′-CAGGGATCAAAGCTGTAGGC-3′ for TNF-α , F 5′-GAAATCCCATCACCATCTTCCAGG …
WebNov 1, 2024 · RNA concentration was determined by NanoDrop OneC spectrophotometer (Thermo Scientific, USA). PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA Corporation, Japan) was used to reverse transcribe RNA into cDNA. qRT -PCR was analyzed using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (TAKARA … WebOct 7, 2024 · The target gene and β-actin expression levels were detected using the TB Green ® Premix Ex TaqTM II Kit (TaKaRa, Tokyo, Japan). The reaction was performed …
WebSep 21, 2024 · The purity of total RNA was evaluated using spectrophotometry. cDNA was then synthesized using a PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa, China). RT-qPCR was performed using TB Green® Premix Ex TaqTM II (TaKaRa, China) according to the manufacturer’s instructions. WebBacterial cells were harvested after induction for 20, 40, 60 and 90 min. Total RNA was extracted and reverse transcribed to cDNA, and real-time PCR was performed using TB Green TM Premix Ex TaqTM II kit (TaKaRa) on the CFX Connect (Bio-Rad) with primers listed in Supplementary Table S3.
WebJan 8, 2007 · The Ex Taq™ HS is an enzyme for hot start PCR which utilizes an anti-Taq antibody. The SYBR® Premix Ex Taq™ mix also contains dNTPs, Mg2+ and a newly …
WebJan 6, 2024 · RT-PCR（ TaKaRa TB Green Premix Ex Taq TM Ⅱ Cat#RR820A LOT#AJ60874A ） TB Green Premix Ex Taq （2X）（Tli RNaseH Plus），Bulk 10ul. … humer bau peuerbachWebYou need to enable JavaScript to run this app. humentum ukWebDec 9, 2024 · Then, total RNA was used to compound cDNA using the Prime ScriptTM RT reagent kit (Takara) according to the manufacturer's protocol. Quantitative real‐time PCR was performed to measure the messenger RNA levels of Col‐1, α‐SMA, IL‐1b, IL‐6, TGF‐b1, FN14, USP4 using TB Green Premix Ex TaqTM II (Takrara) in ABI7500 Fast … humer adamWebJul 16, 2024 · Using GAPDH as an internal reference gene, RT-qPCR was performed using TB Green TM Premix Ex TaqTM II (Takara). The reaction system was as follows: TB Green Premix Ex Taq II (Tli RNaseH Plus) 10 μL, PCR forward primer (10 μM) 0.8 μL, PCR reverse primer (10 μM) 0.8 μL, DNA (< 100 ng) 2 μL, and sterilized water 6.4 μL. humer adultihumer conjugaison imperatifWebSep 30, 2024 · The total RNA of the third and fourth leaves was extracted using an EASY spin rapid plant RNA extraction kit (Aidlab, China). Reverse transcription (RT) was then performed using an M5 Sprint qPCR RT kit with genomic DNA remover, following which real-time qPCR was performed using TB Green ® Premix Ex TaqTM II (Takara, China) … humer baumarkt peuerbachWebReactions were performed in triplicate using an Mx3005P qPCR System (Stratagene Agilent, CA, USA) with the TB Green ® Premix Ex TaqTM II Kit (TaKaRa, Dalian, China) as we previously reported. 25 Relative mRNA expression levels were determined by comparison with expression levels in the reference strain K. pneumoniae ATCC13883. humer bau gmbh