Mitomycin c prophage

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August undefined, 2024

WebThe results of the H. aquamarina phage (HAP-1) mitomycin C prophage in- duction experiments are shown in Fig. 1. In the mitomycin C-treated cultures, lytic activity was observed, ... Web19 sep. 2016 · Although activation of the C. pasteurianum prophage occurred spontaneously in the aforementioned analysis, prophage excision can be artificially induced using ultraviolet irradiation or mitomycin ...

Genomic and Genetic Analysis of Bordetella Bacteriophages …

WebInduction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. … lgim stewardship report

Frontiers Detecting Lactococcus lactis Prophages by …

WebMitomycin C (MMC) acts as a prototype for drugs with bioreductive alkylation. It is mainly found to be active under anaerobic conditions. MMC exhibits a vast clinical … WebMitomycin C-treated bacteria resumed DNA synthesis also when infected with either T 3 or T 5 phage. 3. 3. Although mitomycin C did not affect the infectivity of free phage, the reproduction of phage was progressively impaired with increasing concentrations of the antibiotic. Phage-infected bacteria in contact with high levels of mitomycin C ... WebThe frequency of prophage carriage was tested in a collection of 108 clinical isolates of Streptococcus pneumoniae . A PCR-based assay was developed to allow classification of the prophage into the three groups recently identified according to genome lgim synthetic credit fund

How to propagate & concentrate the prophage after induction …

Prophage Induction Causes Geobacter Electroactive Biofilm Decay.

Web21 dec. 2024 · In contrast, in the presence of mitomycin C, the fitness benefits of ARGs relative to prophages were less pronounced or even absent, and lysogen fitness was primarily determined by prophage carriage (in Cluster 1 there was a higher benefit of prophages than ARGs, and mitomycin C was present in most of these treatment … WebExposure to mitomycin C induces no DNA breakdown in these mutants (Boyce & Howard-Flanders, 1964). A recA uvr A double mutant was first constructed by Howard-Flander & Boycse (1966). The ultraviolet dose for 37 % survival of the double mutant is 0-2 erg/mm2, corresponding approximately to one dimer per bacterial genome (Howard-Flanders, 1968). lgim thematic etfWeb6 dec. 2024 · Mitomycin C acts on DNA in three possible ways: promoting (a) cross-linking events, (b) alkylation and not necessarily cross-linking, and (c) strand breakage. … lgim switchboard

"Web24 aug. 2024 · When the culture reached an OD 600nm of 0.15–0.25, mitomycin C was added to 0.3 μg/ml, inverted several times for gentle mixing, and incubated at 37 °C in an anaerobic chamber for 15–20 h. " - Mitomycin c prophage

Mitomycin c prophage

Mitomycin C: mechanism of action, usefulness and limitations

WebTranscript levels of prophage-encoded genes in strain MGAS315 during culture with mitomycin C, hydrogen peroxide, and D562 epithelial cells relative to transcript levels under control conditions. Gene names are listed on the y axis. WebThe mitomycins are antitumor antibiotics that are under investigation now for more than 30 years. Mitomycin C (MMC) is the best investigated subtype. It serves as a prototype for drugs with bioreductive alkylation, which is a unique feature of this class. MMC is mainly active under anaerobic circumstances.

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Web19 jul. 2024 · Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a … WebVibrio vulnificus is a bacterium known to cause fatal necrotizing soft tissue infection in humans. Here, a remarkable therapeutic effect of hyperbaric oxygen (HBO) on V. vulnificus infection provoked by its injection into mouse footpads is described. HBO was shown to be bactericidal to this bacterium in vitro as well as in the infected tissue. The bactericidal …

WebChemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to … WebMitomycin C (215a) shows a potent activity against a wide spectrum of tumors and bacteria. In spite of its high toxicity it is extensively used in chemotherapy especially in treating gastrointestinal cancers 〈B-80MI 801-01〉. For synthetic chemists the structure of mitomycins presents a formidable challenge.

Web5 aug. 2003 · Parasite adaptation to dynamic host characteristics is a common theme in biology. We recently identified a unique mechanism of adaptation that governs the interactions between a group of bacterial pathogens belonging to the Bordetella genus and a family of bacteriophages that infect them ().As pathogens of numerous mammalian … Web17 jun. 2024 · Previous data suggest that gene exchange may occur in Yersinia. Only scarce information exists about temperate phages of Y. enterocolitica, even though many prophage sequences are present in this species. We have examined 102 pathogenic Y. enterocolitica strains for the presence of inducible prophages by mitomycin C treatment.

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Web16 jun. 2024 · Gene Map and Features of Φstx 2dact prophage. Lambdoid phage Φ1720a-02 was originally isolated from EC1720a-02, a STEC strain found in packaged ground beef [].To create the novel C. rodentium-mediated mouse model of EHEC infection, C. rodentium DBS100 (also known as C. rodentium strain ICC 168 (GenBank accession number … lgim thematic investing blogWebDNA synthesis is selectively inhibited in Salmonella typhimurium by treatment with the antibiotic mitomycin C. These inhibited cells will, however, produce active particles on infection with phage P22. Genetic recombination between marked phages is not affected, but the frequency of lysogenization is drastically reduced under these conditions. lgim sustainable dc property fundWebThe genome of strain MGAS315 has six prophage-like elements, but it is unknown if they can be induced. Mitomycin C is a DNA-damaging agent and is known to activate recA … lg ims todayWebTo understand the function of these prophages, cultures of TM1040 were treated with mitomycin C to induce the production of viral particles. A significant increase in viral counts and a decrease in bacterial counts when treated with mitomycin C suggested that prophages were induced from TM1040. lgim sustainable property fundWeb9 apr. 2024 · MITOMYCIN C, a newly isolated antibiotic, is receiving special attention because of its anti-neo-plastic activity as well as its selective inhibitory action on the … lgim thematic fundsWebWRO~OGY 13, 493-499 (1961) Effect of Mitomycin C on Interactions between Temperate Phages and Bacteria~ MYRON LEVINE Biology Department, Broot~haven National Laboratory, Upton, New York Accepted December 21, 1960 DNA syttthesis is selectively inhibited in Salmonella typhimurium by treatment with the antibiotic … lgim voting rationaleWeb10 jan. 2024 · Using viral and bacterial mutants in our EHEC disease mouse model, we showed that whereas an inducing signal needed to begin viral replication was essential for lethal disease, virus production was not: … lgim synthetic leveraged credit fund