Cisplatin h460
Web检测H460细胞中p-Akt蛋白的表达。将细胞接种于6孔板中,加入不同浓度(10、20、30 μmol/L)芝麻素处理12 h后,在每孔细胞中加入100 μL的蛋白裂解液后,采用BCA试剂盒测定蛋白浓度,计算每个泳道的上样量为70 μg总蛋白,计算5× loading buffer量及超纯水量,定 … WebApr 10, 2024 · Microarray data from resistant and sensitive cisplatin H460 lung cancer cell line: Data processing: The analysis and visualization of the microarray data was performed using Agilent Cytogenomic 3.0.1.1. Submission date: Apr 11, 2024: Last update date: Apr 10, 2024: Contact name: Carlos Rodriguez Antolin: Organization name
Cisplatin h460
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WebJul 28, 2024 · Cisplatin-induced cytotoxicity in human lung cancer cells. a MTT assay revealed the significant reduction in the relative cell viability in lung cancer H460 cells after treatment with 10–100 µM cisplatin for 24 h. b Increased level of apoptosis was noted after culturing with cisplatin at 25–100 µM. c Bright blue fluorescence of Hoechst33342, … WebJan 26, 2012 · The cisplatin resistant clone (H460/cis) of H460 cells was established by exposing the cells with gradually increasing concentrations of cisplatin until chemoresistance acquisition was elucidated by MTT, Hoechst 33342 staining and comet assays. Degree of autophagosome formation and level of LC3 marker were evaluated by …
WebOct 31, 2024 · Generation of Cisplatin-Resistant Lung Cancer Cell Lines A549, H460, H661, and H1299 lung cancer cells were exposed to cisplatin according to the IC50 value for 72 h. The medium was removed, and the cells were cultured in normal medium until the cells fully recovered. WebFlap endonuclease 1 (FEN1) plays a crucial role in both DNA replication and damage repair. In this study, we investigated FEN1 expression and its clinical-pathological significance in non-small-cell lung cancer (NSCLC). Quantitative reverse PCR and
WebApr 4, 2024 · A pilot study of 12 H460 tumor-bearing xenografts, 4 control (vehicle only) and 8 treated (combination of pemetrexed and cisplatin), revealed tumor growth inhibition of 88% in those treated with pemetrexed and cisplatin relative to vehicle only controls ( Supplemental data Figure S1 ). WebJul 15, 2011 · Effects of Cav-1 on cisplatin-induced H460, H460/Cav-1, and H460/shCav-1 cell death. a Cells were treated with various concentrations of cisplatin (0–250 μM) for 24 h, and then cell viability was determined by XTT assay.b, d Apoptotic cell death was detected by Hoechst 33342 staining assay, counted the cells and performed as % apoptosis.c Cav …
WebApr 26, 2024 · NCI-H460/CDDP and its parental cells were plated in 96-well plates. Cells in 96-well plates were transfected with 2 ng pRL-tk (Promega) and 50 ng p53 reporter plasmid (Addgene) for 24 h with the lipofectamine 3000. Cells were treated with DMSO or BAY-598 at indicated concentrations for 24 h.
WebJun 20, 2024 · H460-Cis and A549-Cis cells were treated with 2 μ g/mL cisplatin to maintain cisplatin resistance character. All human cell lines have been authenticated using short tandem repeat (STR) profiling. Cells were maintained at 37°C in a humidified atmosphere with 5% CO 2. ontario trillium benefit doubledWebThe IC 50 concentrations detected in A549 and H460 cisplatin-resistant cell lines and the parental cell lines. Source publication ABCG2 regulates the pattern of self-renewing … ionic liquids database - ilthermo v2.0WebApr 14, 2024 · To verify the resistance of A549/CR and H460/CR cells, the effect of cisplatin or PGG on viability (Figure 1A) and the PTEN expression level was examined in A549 and H460 parental cells and cisplatin-resistant A549/CR and H460/CR cells using an MTT assay and Western blotting. Cisplatin-resistant cell lines demonstrated a subtle … ionic liquids in separation technologyWeb2 days ago · LUAD cell lines H1299, H460, A549, H1975, CALU1 and CALU6, lung fibroblasts HEL299, lung bronchial epithelial cell line HBEC, normal human bronchial epithelium cell line BEAS2B, kidney embryonic ... ontario trillium benefit eligibility incomeWebCISPLATIN 10 MG INJECTION: Short descriptive text of procedure or modifier code (28 characters or less). The AMA owns the copyright on the CPT codes and descriptions; … ionic liquid-based oral drug delivery systemsWebMay 1, 2024 · Cisplatin-resistant versions of the A549, H1299, and H460 cell lines were generated by continuous drug exposure. The long-term metabolic changes, as well as, the early response to cisplatin treatment were analyzed in both, parental and cisplatin-resistant cell lines. ontario trillium benefit notice amount owingWebLung cancer is a malignant tumor characterized by a rapid proliferation rate, less survivability, high mortality, and metastatic potential. This review focuses on updated research about the clinical application of traditional Chinese medicine (TCM) as an adjuvant therapy to lung cancer treatment and the mechanisms of TCM effect on lung cancer in … ontario trillium benefit overpayment